2018-02-12T14:45:32.000Z2025-05-10 22:48:10
locus
BSU17430
BSU_17430
proteinLength
334
420
geneLength
1002
1263
outlinks
bsu
BSU17430
BSU_17430
Gene
Coordinates
1,875,304 → 1,876,566
1,875,304 1,876,566
The protein
Protein family
GTP1/OBG family (according to Swiss-Prot) Era/Obg family
TRAFAC class OBG-HflX-like GTPase superfamily (with [[protein|Obg]] and [[protein|YyaF]], according to UniProt)
The protein
Paralogous protein(s)
[[protein|Era]], [[protein|Obg]], [[protein|YphC]], [[protein|YsxC]], [[protein|RbgA]], [[protein|YqeH]], [[protein|ThdF]], [[protein|YyaF]]
[[this]]
Biological materials
Mutant
MGNA-A020 (ynbA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/20 NBRP B. subtilis, Japan]
BKE17430 (Δ[[gene|ynbA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE17430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAATGGATGTTCCTTTCTT, downstream forward: _UP4_ATGTAGGAAGGAAATATAAA
BKK17430 (Δ[[gene|ynbA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK17430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAATGGATGTTCCTTTCTT, downstream forward: _UP4_ATGTAGGAAGGAAATATAAA
MGNA-A020 (ynbA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/20 NBRP B. subtilis, Japan]
BKE17430 ([[gene|ynbA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE17430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAATGGATGTTCCTTTCTT, downstream forward: _UP4_ATGTAGGAAGGAAATATAAA
BKK17430 ([[gene|ynbA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK17430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAATGGATGTTCCTTTCTT, downstream forward: _UP4_ATGTAGGAAGGAAATATAAA
Labs working on this gene/protein
[SW|Naotake Ogasawara], Nara, Japan
The protein
[SW|Domains]
Hflx-type G domain (aa 201-363) (according to UniProt)
Biological materials
Expression vector
pGP2845: IPTG inducible expression, purification in ''E. coli'' with C-terminal Strep-tag, in [SW|pGP574], available in [SW|Jörg Stülke]'s lab
pGP2846: expression of Strep-''ynbA'' by [SW|pGP380] in ''B. subtilis'' suitable for [SW|SPINE], available in [SW|Jörg Stülke]'s lab
Biological materials
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab
labs
[SW|Naotake Ogasawara], Nara, Japan